{"data":{"lab":{"puzzle_nid":"2857430","secstruct":"((.(((((....))))).(((((....)).((((....)))).)))..))..(((....))).(((((((....))))))).","rna_type":"single","object":null,"nid":"2857430","created":"1368648880","title":"The GAAA loop","body":"The GAAA tetraloop is one of the most common tetraloop sequences found in nature.  However, in the lab the SHAPE data shows that in a large percentage of cases, the closing base nucleotides are not measured as being tightly bound.  Perusing the CoSSMos database (http://cossmos.slu.edu/), it seems that the nucleotides in the loop can take on quite varied 3D arrangements.  Some of these have the closing base pairs appear to have strong Watson-Crick bindings, but many do not.  The hope for this lab is that we can discover what hairpin base sequences score well in the lab when the loop sequence is GAAA.","uid":"57675","field_puzzle_synthesis_winner_nid":"2389702","exp_phase":"1","exp_phase_start":null,"exp_phase_end":null,"last_round":"0","date":"05/22/2013 11:59 PM","num_synth":"40","affiliation":null,"selection":"user_vote","pending":null,"cover_image":null,"round":1,"synthesized_solutions":[{"title":"GAAA Loop 2z","id":"2872419","puznid":"2857430","score":"93"},{"title":"Which will hold and which will fall apart?","id":"2902540","puznid":"2857430","score":"91"},{"title":"GAAA loop V.2","id":"2885745","puznid":"2857430","score":"91"},{"title":"G3AAA - Brourd - Lab 'The GAAA loop' R1 - Sub 3 (Extra G-C pairs)","id":"2874172","puznid":"2857430","score":"91"},{"title":"Scale","id":"2887512","puznid":"2857430","score":"90"},{"title":"MOD of Ruler by Hoglahoo","id":"2889883","puznid":"2857430","score":"89"},{"title":"GAAA Loop 1z","id":"2872416","puznid":"2857430","score":"89"},{"title":"GAAAH! Loop","id":"2873205","puznid":"2857430","score":"89"},{"title":"G1A - Brourd - Lab 'The GAAA loop' R1 - Sub 1 (GGAAAC closing pairs)","id":"2874165","puznid":"2857430","score":"89"},{"title":"GAAA Loop #1","id":"2905640","puznid":"2857430","score":"89"}],"submitted":"98","num_voters":18,"curr_time":1776069731},"comments":[],"supercomments":[],"num_synthesized":40}}